Killifish

Typical Drosophila vials
Typical Drosophila vials

Housing

Flies are typically kept in plastic or glass containers. I strongly recommend polypropylene plastic vials. Glass containers seem to suffer from condensation to a much greater extent. This can prevent the media from adhering to the vial (allowing it to slip when the vial is inverted), and prevent flies from climbing up the side of the vial.

Re-useability is something that we should all aim for, however fruit fly vials get SO 'glued-on' that it is often very messy and time consuming to clean them effectively, and fly keepers often use disposable cups. However, polypropylene plastic vials with straight sides really are the best choice. The media stays in place better, and if soaked and washed with a bottle brush, can be cleaned and reused easily. Conical flasks are just that bit harder to clean. Such containers are somewhat hard to source, but are available from scientific suppliers online.

Vials can be sealed with sponge (you can make your own - though they are somewhat difficult to cut perfectly; but again available online from scientific suppliers), these are good at keeping out mites, and very convenient. Less good options include: cotton wool (cheap, easy, but only good for vials with small openings. Not reuseable.), filter paper (can be damaged when wet) or netting (cheap, reuseable, can allow mites and interbreeding with wild flies). If you use netting, it is best to keep the flies in a cabinet to keep out other flies.

A funnel is critical when transferring flies
A funnel is critical when transferring flies

Handling fruit flies?

Under laboratory conditions flies need to be moved from container to container without any escape. This is performed by anaesthetising the flies with CO2 or ether. This renders them unconscious for a minute or two without damage. Even fully flighted flies can be easily managed in this way. If you have a cylinder of CO2 handy, perhaps for a planted aquarium, try inverting the vial, and introducing a gentle flow of CO2 by inserting a fine tube past the sponge topper. After just a few seconds the flies will fall unconscious. This is where the consistency of the media is essential. If your flies are kept over sloppy media, when it is inverted, media will slide down the side of the container, sticking to flies and potentially falling out. If you use the Sussex miedia (see fruit fly food recipes) it will stay exactly where it should! Tap the container a couple times, and all the sleeping flies will all fall out. No mess, just flies.

Luckily flightless flies are relatively easy to manipulate without CO2, as long as you work quickly. If you have difficulties, 5-8 minutes in a freezer greatly reduces their mobility. But work quickly, because they will wake up soon!

Always use funnels when moving flies from one vial to another. This will largely prevent escape, and allows a vial to be left 'unlidded' with flies still in it. If any do start to walk back up, a gentle tap soon sends them back to their food!



Fruit fly cultures for sale

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Fruit Fly culture kit. Makes 10 cultures. Free shipping.

Gadsden, Alabama, 359**, UNITED STATES OF AMERICA

$35

This kit contains 10 cups / lids, media that works for both Hydei and Melangaster flies, and excelsior In each 32 oz cup add 1 / 2 cup of media, pour 3 / 4 cup of boiling filtered water, mix well. When media is mixed well add excelsior and cover with lid. Please wait for media to cool before adding in your flies (not included in kit). Keep at room temperature.... more

Golden Hydei Fruit Fly Culture - Hocks Herps LLC

York Haven, Pennsylvania, 173**, UNITED STATES OF AMERICA

$11

This Golden Hydei Fruit Fly Culture from Hocks Herps LLC is perfect for feeding your reptiles. It contains a high volume of nutritious flies that your pets will love. The culture is easy to maintain and comes with all the necessary supplies.

The product is ideal for reptile owners who want to provide their pets with a healthy and tasty diet. The flies are bred in a controlled environment to ensure quality and freshness. Get your Golden Hydei Fruit Fly Culture today and give your ... more

Flightless Fruit Fly Culture 32oz Live Dart Frog Food Reptile Food Live Insects

Middletown, Pennsylvania, 170**, UNITED STATES OF AMERICA

$19

Our Flightless Fruit Fly Culture 32 oz are made with Repashy
Live Flightless Fruit Flies are a great option for feeding small lizards and many species of frogs, especially because of their reproduction cycle. Each culture of flightless fruit flies has a lifespan of about one month, and even after they die, they will continually pupate from eggs laid by the adults in the culture! This will allow you to keep fruit flies for a while, as long as they are taken care of accurately
Oue Fruit ... more

Behavioral Genetics of the Fly, Drosophila Melanogaster, Hardcover by Dubnau,

Jessup, Maryland, 207**, UNITED STATES OF AMERICA

$152

Behavioral Genetics of the Fly, Drosophila Melanogaster, Hardcover by Dubnau, Josh (EDT), ISBN 1107009030, ISBN-13 9781107009035, Like New Used, Free shipping in the US
... more

16 cups and fabric vented lids. Great for fruit fly cultures, plants, insects.

Gadsden, Alabama, 359**, UNITED STATES OF AMERICA

$22

(16) 32ounce cups(16) vented lidsPerfect for fruit fly cultures.... more